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Review: Principles, Evolution, and Applications of Spectrophotometry

Abstract

Spectrophotometry is a cornerstone technique in analytical chemistry and biology, utilized to quantify the concentration of solutes by measuring the amount of light absorbed by a solution. This review outlines the fundamental principles of the Beer-Lambert law, the instrumentation involved in ultraviolet-visible (UV-Vis) spectroscopy, and the diverse applications of this technology in modern science. ebsvpecoth

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